The slow growth of a mob2Δ ras2Δ strain is not due to loss of Ace2 localization or activation. (A) Northern blot of CTS1 and ACT1 RNA from strains Y3020 (wild type [WT]), Y3021 (ras2/ras2), Y3022 (mob2/mob2), and Y3023 (ras2/ras2 mob2/mob2) transformed with plasmids carrying the indicated markers (YEp13, B1373, B2461, or B2462). Normalized values for the levels of CTS1 mRNA are indicated below the Northern blot. (B) Y3018 (ras2Δ mob2Δ [CEN-URA3-RAS2]) containing a plasmid carrying the indicated marker was spotted onto SC and SC plus FOA as for Fig. 1A.