TABLE 5.
ras2Δ and mob2Δ exhibit synthetic bud site selection defects: haploid budding patterna
| Strain genotype | Axial | Bipolar | Random |
|---|---|---|---|
| Wild type | 97 ± 2 | 2.5 ± 2.1 | 0 ± 0 |
| ras2Δ | 89 ± 1 | 11 ± 1 | 0 ± 0 |
| mob2Δ | 94 ± 5 | 5.7 ± 5.2 | 0.5 ± 0.4 |
| ras2Δ mob2Δ | 65 ± 10b | 34 ± 10 | 0.8 ± 0.6 |
| Wild type [vector] | 98 ± 1 | 1.7 ± 1.7 | 0.3 ± 0.5 |
| Wild type (TPK1) | 97 ± 2 | 2.7 ± 2.5 | 0.3 ± 0.5 |
| ras2Δ mob2Δ (vector) | 56 ± 13c | 39 ± 12 | 4.7 ± 1.2 |
| ras2Δ mob2Δ (TPK1) | 92 ± 3 | 8.0 ± 1.4 | 0 ± 1.2 |
a
Haploid strains Y3084 (wild type), Y3085 (ras2Δ), Y3086 (mob2Δ), and Y3087 (ras2Δ mob2Δ), harboring no plasmid or carrying YEp13 (vector) or B1373 (TPK1), were grown to logarithmic phase and scored for position of the first bud by calcofluor-white staining as described in Materials and Methods. Numbers represent the average percentages (±standard deviations) of three independent experiments in which at least 100 cells were counted.
b
P < 2 × 10−7 for percent nonaxial buds = percent wild-type nonaxial buds and for percent nonaxial buds = percent mob2Δ nonaxial buds.
c
P < 6 × 10−8 for percent nonaxial buds = percent wild-type nonaxial buds.