FIG. 5.

Western blot analysis of AOX proteins in PaCox17::ble strains grown in rich medium or rich medium with 250 μM copper sulfate. The juvenile wild-type (WT) strains, mutant ex1, mutant grisea on rich medium, or on mutant grisea on medium containing 250 μM additional copper serve as controls. Mitochondria were isolated, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and blotted onto nitrocellulose. PaAOX was detected with monoclonal mouse antibodies against the AOX of Sauromatum guttatum strain Schott (20). An antibody against mitochondrial ATPase subunit β was used as loading control.