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. 2004 Feb;3(1):100–107. doi: 10.1128/EC.3.1.100-107.2004

FIG. 4.

FIG. 4.

The KCl sensitivity of the ppz1 ppz2 strain is exacerbated by the further disruption of MPK1. The indicated strains (W303-1A, LY190, LY139, and LY196) were grown to saturation in YPD plus 1 M sorbitol (SORB.), serially diluted in sterile 1 M sorbitol, and spotted onto YPD plates containing the indicated amount of KCl or sorbitol. Images were taken after 2 to 3 days of incubation at 28°C. Identical results were obtained in two separate experiments. Identical results were also observed in minimal media (data not shown).