Fig 1.

The M. leprae targets of dapsone (ML0224, folP1), rifampin (ML1891c, rpoB), and fluoroquinolones (ML0006, gyrA). The partial nucleotide (upper) and corresponding amino acid (lower) sequences containing the drug resistance-determining regions (DRDR) of the target genes are presented. The nucleotides and the amino acid numbers are shown with reference to the open reading frames of the genes for the M. leprae TN strain as found in the Leproma website (http://genolist.pasteur.fr/Leproma/). The codons/amino acids implicated in drug resistance are shown within boxes. The primer sequences selected for real-time PCR-HRM are underlined.