Fig 2.

Real-time PCR-HRM analysis for detection of mutations in M. leprae drug resistance-determining regions (DRDR assays). Representative real-time-PCR normalized melt curves (A) and differential curves (B) for M. leprae strains analyzed at the DRDRs of folP1, rpoB and gyrA are shown. The green color was assigned to the NHDP63 strain serving as the wild type for each DRDR. The mutant or mixed-genotype strains are shown in red, orange, and blue. The genotypes of the mutants are indicated within parentheses next to the sample name.