Table 1.
Primer sequences for SNP typing by real-time PCR-HRM analysis
| SNP target and locationa,b | Primer name | Primer locationb | Primer sequence (5′–3′) |
|---|---|---|---|
| Locus 1: 14,676 | HRM14F | 14601–14621 | TGAACAGTCTCGTAACCGTG |
| HRMM14Ra | 14721–14701 | CAATGCATGCTAGCCTTAATG | |
| Locus 2: 1,642,875 | HRM16F | 1642813–1642836 | CTCGTCACAAATCCGAGTTTGAAT |
| HRM16R | 1642925–1642902 | GTAGTAGTCTTCCAAGTTGTGGTG | |
| Locus 3: 2,935,685 | HRM29F | 2935599–2935616 | TGGTGTCGGTCTCCATCC |
| HRM29Ra | 2935716–2935699 | ACCGGTGAGCGCACTAAG |
a
Data are from the study by Monot et al. (28).
b
Data correspond to the M. leprae TN genome sequence (http://genolist.pasteur.fr/Leproma/).