Table 5.
Comparison of real-time PCR CT values and estimates of starting quantity
| Sample typea | Strain (quantity) | SNPb |
|||||
|---|---|---|---|---|---|---|---|
| Locus 1 |
Locus 2 |
Locus 3 |
|||||
| CT | SQ (pg) | CT | SQ (pg) | CT | SQ (pg) | ||
| ADML | NHDP63 (1 ng) | 21.95 | 1,000.00 | 21.97 | 1,000.00 | 21.01 | 1,000.00 |
| NHDP63 (100 pg) | 25.40 | 100.00 | 25.34 | 100.00 | 24.24 | 100.00 | |
| NHDP63 (10 pg) | 29.09 | 10.00 | 28.73 | 10.00 | 27.76 | 10.00 | |
| NHDP63 (1 pg) | 32.70 | 1.00 | 32.24 | 1.00 | 31.14 | 1.00 | |
| NHDP63 (0.1 pg) | 36.50 | 0.10 | 35.75 | 0.10 | 34.43 | 0.10 | |
| Thai-53 | 27.14 | 26.40 | 27.42 | 20.71 | 26.09 | 29.48 | |
| 3039 | 27.16 | 25.97 | 27.15 | 24.60 | 26.16 | 28.16 | |
| BR4923 | 27.25 | 25.36 | 27.18 | 24.36 | 26.24 | 26.79 | |
| MFP | Airaku-2 | 26.35 | 58.92 | 26.30 | 53.48 | 25.25 | 53.64 |
| Airaku-3 | 27.08 | 37.60 | 27.14 | 30.74 | 25.90 | 34.52 | |
| Amami | 26.13 | 67.50 | 25.85 | 72.29 | 25.01 | 63.49 | |
| Hoshizuka-4 | 29.95 | 6.47 | 29.52 | 6.19 | 28.73 | 5.00 | |
| Kusatsu-3 | 29.17 | 10.40 | 29.32 | 7.11 | 28.00 | 8.22 | |
| Kusatsu-6 | 27.30 | 32.85 | 27.16 | 30.02 | 26.08 | 30.61 | |
| Ryukyu-6 | 28.17 | 19.35 | 27.90 | 18.30 | 26.58 | 21.74 | |
| Zensho-2 | 27.28 | 33.35 | 27.73 | 20.67 | 25.62 | 41.90 | |
| Zensho-4 | 27.70 | 25.73 | 27.26 | 28.14 | 26.41 | 24.31 | |
| Zensho-5 | 27.82 | 23.87 | 27.65 | 21.76 | 27.02 | 16.04 | |
| Zensho-9 | 27.38 | 31.21 | 27.12 | 30.92 | 26.35 | 25.33 | |
| Zensho-15 | 28.68 | 14.11 | 28.45 | 12.79 | 27.64 | 10.52 | |
| Gushiken | 25.90 | 77.88 | 25.96 | 67.15 | 24.39 | 97.04 | |
| Hoshizuka-5 | 28.04 | 20.87 | 28.03 | 16.79 | 26.59 | 21.60 | |
| Indonesia-1 | 27.86 | 23.70 | 27.40 | 25.64 | 25.93 | 33.77 | |
| Korea-3-2 | 27.93 | 22.40 | 28.05 | 16.58 | 26.46 | 23.56 | |
| Thai-53 | 27.42 | 30.50 | 27.32 | 26.91 | 26.19 | 28.33 | |
| Thai-311 | 25.95 | 75.25 | 25.88 | 70.67 | 24.84 | 71.41 | |
a
ADML, armadillo-derived M. leprae; MFP, mouse footpad-derived M. leprae.
b
Percent efficiency, correlation of coefficient of determination (R2), and slope are 90.3, 0.994, and 3.579 for locus 1, 91.7, 0.998, and 3.538 for locus 2, and 91.7, 0.999, and 3.539 for locus 3. For starting quantity (SQ) determinations, all DNA templates were tested in triplicate for each target and quantitated according to the NHDP63 DNA standard curve.