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Scheme for real-time PCR-HRM assay for M. leprae SNP typing showing the expected real-time PCR-HRM cluster patterns for the three loci which generate four SNP types
| SNP type | Allelea/HRM clusterb |
||
|---|---|---|---|
| Locus 1 | Locus 2 | Locus 3 | |
| 1 | C/1 | G/2 | A/2 |
| 2 | C/1 | T/1 | A/2 |
| 3 | C/1 | T/1 | C/1 |
| 4 | T/2 | T/1 | C/1 |
The SNP alleles are indicated (28).
The NHDP63 allele is assigned to cluster 1 and the alternative allele to cluster 2.
