Table 7.
Results of real-time PCR-HRM assay for M. leprae SNP typing of MFP-LRC and armadillo-derived reference strains based on the cluster pattern defined in Table 6
| Sample type | Strain | HRM cluster |
SNP type | ||
|---|---|---|---|---|---|
| Locus 1 | Locus 2 | Locus 3 | |||
| MFP | Airaku-2 | 1 | 2 | 2 | 1 |
| Airaku-3 | 1a | 2a | 2 | 1b | |
| Amami | 1 | 1 | 1 | 3 | |
| Hoshizuka-4 | 1 | 1 | 1 | 3 | |
| Kusatsu-3 | 1 | 1 | 1 | 3 | |
| Kusatsu-6 | 1 | 1 | 1 | 3 | |
| Ryukyu-6 | 1 | 1 | 1 | 3 | |
| Zensho-2 | 1 | 1 | 1 | 3 | |
| Zensho-4 | 1 | 1 | 1 | 3 | |
| Zensho-5 | 1 | 1 | 1 | 3 | |
| Zensho-9 | 1 | 1 | 1 | 3 | |
| Zensho-15 | 1 | 1 | 1 | 3 | |
| Gushiken | 1 | 2 | 2 | 1 | |
| Hoshizuka-5 | 1 | 1 | 1 | 3 | |
| Indonesia-1 | 1a | 2a | 2 | 1b | |
| Korea-3-2 | 1 | 1 | 1 | 3 | |
| Thai-53 | 1 | 2 | 2 | 1 | |
| Thai-311 | 1a | 2a | 2 | 1b | |
| ADML | Thai-53 | 1 | 2 | 2 | 1 |
| 3039 | 1 | 1 | 2 | 2 | |
| BR4923 | 2 | 1 | 1 | 4 | |
| NHDP63 | 1 | 1 | 1 | 3 | |
a
Sequences of amplicons were verified.
b
The SNP types are different from those reported in a previous study (22).