Table 1.

Primers used in this study

Species	Target gene	Fragment size (bp)a	Outer primerb		Inner primer		Reference
			Name	Sequence (5′–3′)	Name	Sequence (5′–3′)
“Ca. Neoehrlichia mikurensis”	16S rRNA	488*	EC9	TAC CTT GTT ACG ACT T	IS58-62f	GGA ATA GCT GTT AGA AAT GAC A	7
			EC12A	TGA TCC TGG CTC AGA ACG AAC	IS58-594r	CTA TCC TCT CTC GAT CTC TAG TTT
	groESL	315*	gro607F1	GAA GAT GCT GTD GGA TGT ACT GC	gro667F2	ATT ACT CAG AGT GCT TCT CAG TG	20
			gro1294R1	AGT GCT TCA CCT TCT ACA TCT TC	gro1121R2	TGC ATR CCR TCA GTT TTT TCA AC
A. phagocytophilum	p44	334	MSP3F	CCA GCG TTT AGC AAG ATA AGA G			26
			MSP3R	GCC CAG TAA CAA CAT CAT AAG C
Borrelia sp.	16S rRNA	659*	16S1A	CTA ACG CTG GCA GTG CGT CTT AAG C	16S2A	AGT CAA ACG GGA TGT AGC AAT ACA	15
			16S1B	AGC GTC AGT CTT GAC CCA GAA GTT C	16S2B	GGT ATT CTT TCT GAT ATC AAC AG

^a*, The product size was obtained using the inner primers.

^bBoldface letters indicate modifications of the published primer.