Table 2.
HAdV types with corresponding LoD, Tm range, and mutations in probe region
| HAdV typeb | LoD for representative type of whole virus (TCID50/ml)b | Tm range | Fluorescein probe areaa | LC Red 640 probe areaa |
|---|---|---|---|---|
| 8, 9, 10, 13, 15, 17, 19, 20, 22, 23, 25-30, 32, 33, 36-39, 42-47, 49, 51, 53, 56 | 10−1 (type 19), 100 (type 9) | 61.1–62.0 | CCG TCA GTG AAA ACG TGC | GCT CTC ACA GAT CAC GGG |
| 24 | Not performed | 58.2 | CGG TCA GTG AAA ACG TGC | GCT CTC ACA GAT CAC GGG |
| 31 | Not performed | 56.4 | CCG GCA GTG AAA ACG TTC | GCT CTC ACA GAT CAC GGG |
| 1, 2, 3, 4-7, 11, 14, 21, 34, 35, 50, 55, 57 | 100 (type 4), 101 (types 1, 3) | 55.3–56.7 | CCG TCA GTG AAA ACG TTC | GCT CTC ACA GAT CAC GGG |
| 18, 40, 41, 52 | 10−1 | 55.9–56.6 | CCG TCA GTG AAA ACG TTC | GCC CTC ACA GAT CAC GGA |
| 48, 54 | Not performed | 52.0 | CCG TTA GTG AAA ACG TGC | GCT CTC ACA GAT CAC GGG |
| 16 | 10−1 | 50.9 | CCA TCA GTG AAA ACG TTC | GCT CTC ACA GAT CAC GGG |
| 12 | 103 | 49.9 | CCG TCA GTG AAA ACG TTC | GCC CTT ACA GAT CAC GGG |
a
Mutations in the region targeted by the probes are in bold. There are 2 intervening nucleotides between the donor (fluorescein) probe and acceptor (LC Red 640) probe across which energy transfer occurs.
b
Bolded HAdV types were used to determine the limit of detection for each Tm-based grouping shown above (see Table S3 in the supplemental material for virus strains used). Whole-virus strains were quantitated using the 50% tissue culture infective dose (TCID50).