TABLE 2.
Promoter lacZ fusions constructed for this studya
| Plasmid | lacZ vector | Promoter | Length of the cloned upstream region (bp) | Primer pair |
|---|---|---|---|---|
| pBM4270 | YEp357R | MIG2 | 1,459 | OM2392-OM2458 |
| pBM4270 | YEp357R | STD1 | 996 | OM2799-OM2800 |
| pBM4273 | YEp357R | YOR062C | 1,180 | OM2717-OM2718 |
| pBM4379 | YEp357R | MIG3 | 713 | OM2563-OM2564 |
| pBM4381 | YEp357R | YGL157W | 646 | OM3534-OM2566 |
| pBM4381 | YEp357R | YNL234W | 418 | OM3446-OM3447 |
| pBM4515 | YEp357R | YGL039W | 756 | OM3633-OM3634 |
| pBM4522 | YEp357R | HSF1 | 813 | OM3676-OM3677 |
| pBM4487 | YEp367R | SNF3 | 832 | OM3882-OM3883 |
| pBM4510 | YEp367R | PHM8 | 934 | OM3089-OM3090 |
| pBM4511 | YEp367R | AQR1 | 1,087 | OM4065-OM4066 |
| pBM4292 | pBM4088 | MTH1 | 1,476 | OM2812-OM3191 |
| pBM4296 | pBM4088 | MRK1 | 626 | OM2844-OM3190 |
| pBM4346 | pBM4088 | MIG2 | 1,459 | OM2875-OM2458 |
| pBM4500 | pBM4088 | VID24 | 686 | OM3448-OM3449 |
| pBM4501 | pBM4088 | HXT5 | 1,500 | OM3318-OM3441 |
| pBM4502 | pBM4088 | AHP1 | 967 | OM3088-OM3981 |
| pBM4512 | pBM4088 | CIT2 | 1,024 | OM4067-OM4068 |
| pBM4513 | pBM4088 | HOR2 | 1,092 | OM4069-OM4070 |
| pBM4514 | pBM4088 | PFK27 | 1,021 | OM3979-OM4071 |
| pBM4516 | pBM4088 | SKS1 | 1,340 | OM4072-OM4073 |
a
The gap repair procedure used for construction of the lacZ fusions is described in Materials and Methods. We also tested a YKR075C-lacZ fusion plasmid, pBM3469, and an MIG1-lacZ fusion plasmid, pBM3091, as described previously (43). Sequences of the primers are available upon request.