TABLE 4.
Newly identified targets of Rgt1 with unknown functionsa
| Promoter fusion | Activity of lacZ reporter
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Wild type
|
rgt1Δ strain
|
RGT2-1 strain
|
SNF3-1 strain
|
||||||
| Gal | Raf | Glu | Gal | Glu | Gal | Glu | Gal | Glu | |
| YKR075c-lacZ | 612.8 | 2,985.6 | 183.5 | 10,324.8 | 1,925.5 | 1,434.3 | 532.0 | 4,877.2 | 1,215.5 |
| YOR062c-lacZ | 44.7 | 88.8 | 305.0 | 540.2 | 200.8 | 139.3 | 258.4 | 307.1 | 208.2 |
| YGL157w-lacZ | 98.4 | 181.9 | 704.9 | 1,094.0 | 456.6 | 200.8 | 569.7 | 589.6 | 592.7 |
| YNL234w-lacZ | 2.5 | 5.3 | 12.9 | 19.4 | 12.3 | 6.4 | NT | 14.7 | NT |
Strains transformed with plasmids carrying the indicated lacZ fusions (Table 1) were pregrown in galactose medium selective for the plasmid, inoculated into media with the indicated carbon source at an OD600 of approximately 0.1, and grown until mid-log phase (usually 5 to 6 h). Activities of lacZ reporters (in nanomoles of ONPG per milligram of protein per minute) were measured in cellular extracts prepared from the following cultures: BY4741 (FM391) (wild type), FM557 (rgt1Δ), YM6546 (RGT2-1), and YM6549 (SNF3-1). Plasmids used were pBM3469 (43) (pYKR075C-lacZ), pBM4273 (pYOR062C-lacZ), pBM4381 (pYGL157W-lacZ), and pBM4458 (pYNL234W-lacZ). All plasmids carry the 2-micron origin of replication. Raf, raffinose; Glu, glucose; NT, not tested.