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. 2012 Mar;50(3):938–942. doi: 10.1128/JCM.06221-11

Table 1.

Recovery of P. aeruginosa biofilm with each test solutiona

Tube portion sampled (n = 7) Bacteria recovered from each tube portion (log10 CFU/cm2)b
Letheen broth
Saline solution (0.9%)
Sterile water
Bacteria recovered Ratio Bacteria recovered Ratio Bacteria recovered Ratio
Tube portion
    1 7.50 0.91 7.11 0.82 6.48 0.85
    2 7.79 0.95 7.09 0.81 6.88 0.90
    3 7.67 0.93 6.77 0.77 6.56 0.86
    4 7.50 0.91 7.11 0.82 6.59 0.86
    5 7.53 0.92 7.12 0.82 6.01 0.79
    6 7.83 0.95 7.12 0.82 6.02 0.79
    7 7.80 0.95 7.31 0.84 6.50 0.85
Mean ± SD 7.66 ± 0,15 0.93 ± 0.02 7.09 ± 0.16 0.81 ± 0.02 6.43 ± 0.32 0.84 ± 0.04
a

The mean logarithmic counts in control samples (n = 3) for biofilm ± the standard deviation (log10 CFU/cm2) for Letheen broth, saline solution, and sterile water were 8.20 ± 0.06, 8.71 ± 0.28, and 7.64 ± 0.21, respectively. The percentages of biofilm recovery (CFU/cm2) in these control samples for Letheen broth, saline solution, and sterile water were 30.1 (4.8 × 107/1.6 × 108), 2.2 (1.3 × 107/5.8 108), and 7.1 (3.3 × 106/4.7 × 107), respectively. The percent biofilm recovery for each sampling solution was calculated using the mean bacterial count (CFU/cm2) obtained for the seven portions with the test solution as the numerator and the mean bacterial count (CFU/cm2) recovered from the three tube control portions as the denominator.

b

The logarithmic ratio for each tube portion was calculated as the bacterial count (log10 CFU/cm2) recovered by the tested solution in each tube portion as the numerator and the mean bacterial count (log10 CFU/cm2) recovered from the three tube control portions as the denominator.