Table 1.
Tube portion sampled (n = 7) | Bacteria recovered from each tube portion (log10 CFU/cm2)b |
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---|---|---|---|---|---|---|
Letheen broth |
Saline solution (0.9%) |
Sterile water |
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Bacteria recovered | Ratio | Bacteria recovered | Ratio | Bacteria recovered | Ratio | |
Tube portion | ||||||
1 | 7.50 | 0.91 | 7.11 | 0.82 | 6.48 | 0.85 |
2 | 7.79 | 0.95 | 7.09 | 0.81 | 6.88 | 0.90 |
3 | 7.67 | 0.93 | 6.77 | 0.77 | 6.56 | 0.86 |
4 | 7.50 | 0.91 | 7.11 | 0.82 | 6.59 | 0.86 |
5 | 7.53 | 0.92 | 7.12 | 0.82 | 6.01 | 0.79 |
6 | 7.83 | 0.95 | 7.12 | 0.82 | 6.02 | 0.79 |
7 | 7.80 | 0.95 | 7.31 | 0.84 | 6.50 | 0.85 |
Mean ± SD | 7.66 ± 0,15 | 0.93 ± 0.02 | 7.09 ± 0.16 | 0.81 ± 0.02 | 6.43 ± 0.32 | 0.84 ± 0.04 |
The mean logarithmic counts in control samples (n = 3) for biofilm ± the standard deviation (log10 CFU/cm2) for Letheen broth, saline solution, and sterile water were 8.20 ± 0.06, 8.71 ± 0.28, and 7.64 ± 0.21, respectively. The percentages of biofilm recovery (CFU/cm2) in these control samples for Letheen broth, saline solution, and sterile water were 30.1 (4.8 × 107/1.6 × 108), 2.2 (1.3 × 107/5.8 108), and 7.1 (3.3 × 106/4.7 × 107), respectively. The percent biofilm recovery for each sampling solution was calculated using the mean bacterial count (CFU/cm2) obtained for the seven portions with the test solution as the numerator and the mean bacterial count (CFU/cm2) recovered from the three tube control portions as the denominator.
The logarithmic ratio for each tube portion was calculated as the bacterial count (log10 CFU/cm2) recovered by the tested solution in each tube portion as the numerator and the mean bacterial count (log10 CFU/cm2) recovered from the three tube control portions as the denominator.