Table 1.

Methods and reagents used for serotyping of Haemophilus influenza and serogrouping of Neisseria meningitidis

Laboratory	Serotyping of H. influenzae		Serogrouping of N. meningitidis
	Method	Source, reagent, or reference(s)	Method	Source, reagent, and/or reference(s)
1	Slide agglutination	Difco Laboratories	Real-time PCRa	27
2	Slide agglutination	Remel (Thermo Fisher)	Slide agglutination	Antisera provided by the National Microbiology Laboratory;
			PCRb	8, 9, 19, 36
3	PCR	15, 18	Slide agglutination	Antisera provided by the National Microbiology Laboratory
4	Slide agglutination	Difco Laboratories	Latex agglutination	Slidex (Pasteur-Merieux), Wellcogen (Remel), Pastorex meningitis kit (Bio-Rad Laboratories)
			Coagglutination	In-house group-specific rabbit antisera; 2
			PCRc	8, 9, 36
5	Slide agglutination	Difco Laboratories	Slide agglutination	In-house-produced rabbit antisera; 3
	PCR	15	PCRd	5, 8, 9, 36

^aPCR targeting the sacB gene of serogroup A, the siaD genes of serogroups B and C, the synF gene of serogroup Y, the synG gene of serogroup W135, and the xcbB gene of serogroup X.

^bPCR targeting the sacB and mynC genes of serogroup A, the siaD genes of serogroups B and C, the synF gene of serogroup Y, and the synG gene of serogroup W135.

^cPCR targeting the sacB and mynC genes of serogroup A, the siaD genes of serogroups B and C, the synF gene of serogroup Y, and the synG gene of serogroup W135.

^dPCR targeting the sacB gene of serogroup A, the siaD genes of serogroups B and C, the synF gene of serogroup Y, the synG gene of serogroup W135, and unique regions of the ctrA genes specific for serogroups 29E, X, and Z.