Table 4.
Results for pooling specimens positive for influenza with specimens positive for other respiratory viruses
| PCR markera |
CT value for: |
|
|---|---|---|
| 2009 A/H1N1 specimen | Poolb | |
| InfA | 25 | 18 ± 0 |
| swInfA | 26 | 26 ± 0 |
| swH1 | 28 | 29 ± 1 |
| InfB | NA | 22 ± 0 |
InfA, influenza A virus matrix (M) gene target for detecting any influenza A virus; swInfA, influenza A virus nucleoprotein (NP) gene from swine for detecting swine influenza virus in the genetic lineage of 2009 pandemic influenza A (H1N1); swH1, highly conserved region of hemagglutinin (HA) gene for detecting the 2009 pandemic influenza A virus (H1N1), subtype H1; InfB, influenza B virus nonstructural protein (NS) for detecting any influenza B virus.
Representative PCR results (crossing threshold, CT) for pool of 10 specimens containing one that is positive for the 2009 A/H1N1 virus and others positive for other respiratory viruses. The pool contains one specimen positive for 2009 A/H1N1, one for human coronavirus OC43, one for human metapneumovirus, one for rhinovirus, one for seasonal influenza B virus, one for seasonal influenza A/H3 virus, three for seasonal influenza A/H1 viruses, and one negative specimen. PCR was performed in triplicate for the pool sample. Data are the mean CT values of the pool with the standard deviations of the triplicate PCR results. NA, not applicable.