Table 5.
Results for positive specimens from multisite studya
| Sample | Site |
CT value for: |
|||
|---|---|---|---|---|---|
| InfA | swInfA | swH1 | RP | ||
| 9 | A | 24 ± 1 | 25 ± 1 | 28 ± 0 | 26 ± 1 |
| B | 27 ± 2 | 27 ± 4 | 28 ± 0 | 27 ± 1 | |
| C | 24 ± 0 | 24 ± 0 | 28 ± 0 | 25 ± 0 | |
| 10 | A | 24 ± 2 | 26 ± 2 | 28 ± 0 | 25 ± 1 |
| B | 25 ± 2 | 27 ± 3 | 29 ± 1 | 27 ± 1 | |
| C | 24 ± 0 | 25 ± 0 | 32 ± 0 | 24 ± 0 | |
| 44 | A | 24 ± 1 | 25 ± 1 | 28 ± 1 | 25 ± 1 |
| B | 26 ± 2 | 25 ± 2 | 28 ± 0 | 25 ± 1 | |
| C | 23 ± 0 | 24 ± 0 | 28 ± 0 | 23 ± 1 | |
Specimens from pools that tested positive for the 2009 H1N1 influenza virus were re-extracted and retested by PCR individually. The average PCR results of samples 9, 10, and 44 reported by sites A, B, and C are represented above. Samples 9 and 10 are from pool A, and sample 44 is from pool E. Samples 1 to 8 from pool A were negative for influenza A virus (InfA), swine influenza A virus (swInfA), and swine influenza virus H1 (swH1) and were positive for RNase P (RP). Samples 41 and 45 from pool E were positive for influenza A, but negative for the swInfA and swH1 markers. InfA, influenza A virus matrix (M) gene target for detecting any influenza A virus; swInfA, influenza A virus nucleoprotein (NP) gene from swine for detecting swine influenza virus in the genetic lineage of 2009 pandemic influenza A virus (H1N1); swH1, highly conserved region of hemagglutinin (HA) gene for detecting the 2009 pandemic influenza A virus (H1N1), subtype H1; RP, human ribonucleoprotein (RP) for detecting human RNase P RNA, used with human clinical specimens to indicate that adequate isolation of nucleic acid resulted from extraction of the clinical specimen. The values are the means and standard deviations of the PCR results from three separate days.