Table 3.
Distribution of Shiga toxin-encoding SBI genotypes among bovine, food, and human clinical STEC O157 isolates from The Netherlands
| Genotypea | Genotyping data |
No. (%) of isolates |
||||
|---|---|---|---|---|---|---|
| stxb | yehVc | wrbAd | Bovine (n = 73) | Food (n = 29) | Human (n = 85) | |
| 1 | stx2 | Occupied | Intact | 24 (32.9) | 11 (37.9) | 42 (49.7) |
| 3 | stx1 + stx2 | Occupied | Occupied | 1 (1.3) | 3 (10.3) | 5 (5.9) |
| 5 | stx2 | Variant-R | Intact | 30 (40.8) | 6 (20.7) | 3 (3.5) |
| 6 | stx1 + stx2 | Variant-R | Intact | 13 (18.4) | 6 (20.7) | 12 (14.2) |
| 11 | stx2 | Intact | Intact | 1 (1.3) | 1 (3.5) | 1 (1.2) |
| 16 | stx1 + stx2 | Occupied | Intact | 2 (2.6) | 1 (3.5) | 6 (7.1) |
| 21 | stx2 | Occupied | Variant-L | 2 (2.6) | 1 (3.5) | 9 (10.1) |
| NTe | 0 (0) | 0 | 7 (8.2) | |||
Stx-encoding bacteriophage insertion site genotypes based on patterns of stx genes and Stx-encoding bacteriophage insertion sites.
PCR detection of stx1 and stx2 genes.
“Occupied” indicates detection of the yehV-Stx1-encoding bacteriophage left and right junctions. “Intact” indicates the detection of neither junction. “Variant-R” indicates that the left junction was detected (occupied) but the right junction was not detected (intact).
“Occupied” indicates bilateral detection of the wrbA-Stx2-encoding bacteriophage junctions. “Intact” indicates the detection of neither junction. Variant-L indicates that the left junction was not detected (intact) but the right junction was detected (occupied).
NT, nontypeable.