Fig 3.

Ang II treatment induced Src-mediated prolonged ERK activation in AT1R/Cl4 cells. (A) Quiescent AT1R/Cl4 cells were treated as described for Fig. 2A. (B) Quiescent AT1R/Cl4 cells were pretreated with PP2 as indicated in Fig. 2B and treated with Ang II (10⁻⁷ M) or vehicle alone for 10 min or 3 h. Cell lysates were subjected to immunoblotting with indicated antibodies. At 48 h after transfection with Src siRNA or scrambled control siRNA, quiescent AT1R/Cl4 cells were exposed to Ang II (10⁻⁷ M) or vehicle alone for 10 min or 3 h. (C) Cell lysates were subjected to immunoblotting with indicated antibodies.