Fig 7.

Ang II-induced phosphorylation of both EGFR at Y845 and caveolin-1 at Y14 was mediated by AT1 receptors in an Src-dependent manner. (A) Phosphorylation of caveolin-1 (Cav) at Y14 was induced by Ang II in a concentration-dependent manner in AT1R/Cl4 cells but not in empty vector-transfected LLCPKcl4 cells (Vector/Cl4). (B) A selective AT1R antagonist, losartan (10⁻⁶ M), but not the selective AT2R antagonist, PD123319 (10⁻⁶ M), inhibited Ang II-induced phosphorylation of EGFR at Y845 and Cav at Y14. (C) An Src kinase inhibitor, PP2 (5 μM), inhibited phosphorylation of Cav at Y14.