Fig 4.

RGMa increases cell-cell adhesion in animal cap cells in a neogenin-dependent and calcium-independent manner. (A to C) Animal cap explants were isolated from stage 9 (St 9) late-blastula embryos and incubated in calcium- and magnesium-free medium (CMFM) for up to 60 min. (A) Control animal cap explants dissociate to form isolated cells in CMFM. (B) RGMa-overexpressing animal cap explants (1.15 ng RGMa mRNA) stayed intact under the same condition. (C) Simultaneous neogenin downregulation (1.15 ng/23 ng RGMa mRNA/NeoMO) rescued the phenotype. Bar, 500 μm.