Fig 6.

vWF and RGD domains are involved in cell migration and adhesion. (A) Schematic of FL-RGMa and two RGMa domain deletion mutants, RGMa-ΔvWF and RGMa-ΔRGD. (B) Schematic of the role of the vWF and RGD domains in RGMa-mediated migration of cell aggregates in animal cap explants. In the absence of the vWF domain, RGMa produced a small number of larger and nonadhesive cells from the animal cap explant. In the absence of the RGD domain, RGMa-mediated cell migration was delayed, but cells spread and formed aggregates by the tail bud stage. (C1 to C3′) Time lapse images of an RGMa-ΔvWF-overexpressing animal cap. (D1 to D3′) Time lapse images of an RGMa-ΔRGD-overexpressing animal cap. C1′ to C3′ and D1′ to D3′ show fluorescent images of C1 to C3 and D1 to D3, respectively. Yellow arrows indicate cells migrating from animal cap explant, and red arrows indicate migrated cells. Insets at C3, C3′, D3, and D3′ show cells that are outside the field of view. Bar, 100 μm.