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. Author manuscript; available in PMC: 2012 Mar 6.
Published in final edited form as: JPEN J Parenter Enteral Nutr. 2008 Jul-Aug;32(4):433–438. doi: 10.1177/0148607108319806

Figure 4.

Figure 4

Effect of ischemia–reperfusion (IR) and luminal nutrients on jejunal production of matrix metalloproteinase-9 (MMP-9). Jejunal gut sacs were created and filled with 60 mM of arginine (Arg), glutamine (Glut), or magnesium sulfate as an osmotic control and subjected to IR as described in the Materials and Methods section. MMP-9 production was assessed by gelatin zymography. Areas of increased MMP-9 activity are detected as areas of increased lucency in the gel at 92 kDa. Representative zymography demonstrates increased MMP-9 activity after IR, which was not modulated by luminal nutrients. The increase in activity was significant when compared with magnesium sulfate controls. There was no increase in MMP-9 activity upon the addition of luminal nutrients in the absence of IR (data not shown). *P < .05 vs magnesium sulfate (ANOVA).