Fig. 1.

Double deficiency of Tab1 and Tab2 phenocopies Tak1 deficiency. (A and B) no-Cre control, epidermal- or intestinal epithelial-specific single- and double-knockout mice, Sk-Tab1^KOTab2^Het, Sk-Tab1^HetTab2^KO, Sk-Tab1Tab2^DKO, In-Tab1^KOTab2^Het, In-Tab1^HetTab2^KO, and In-Tab1Tab2^DKO at postnatal day 5 (skin) or postnatal day 0 (intestine). Epidermal-specific Tak1-deficient mice (Sk-Tak1^KO) at P5 are also shown. Eight double-knockout mice from three litters (skin) and 12 double-knockout mice from five litters (intestine) and their control littermate mice were analyzed and representative mice and the intestines are shown. Each separate photograph shows mice or the intestines from one litter. (C) The dorsal skin sections at P5 or cross-sections of intestine at P0 from control, single-deletion, and double-deletion mice were stained with hematoxylin/eosin. (D) The cross-sections of intestine at P0 were stained with Alcian Blue. (E) Apoptotic cells were detected with TUNEL staining (green). Nuclei were stained by DAPI (blue). Open brackets indicate epidermis. (Scale bars, 40 μm.)