Fig. 4.
Characterization of disulfide-locked TRCF variants. (A) Steady-state ATPase activity of TRCF variants under oxidizing (“ox” superscript) and reducing (“red” superscript) conditions. Shown are averages of turnover numbers, kcat (normalized to wild type), obtained from three independent experiments ± SD. (B) Quantification of total RNA released in RNAP displacement assays. TEC half-lives were estimated from three independent experiments and are shown as the average ± SD. (C and D) SDS/PAGE of CuPh-catalyzed disulfide cross-linking with/without ADP/ATPγS (C) and UvrA-Trunc (D). (E) ATP turnover in the presence of DNA template (Upper) and TECs visualized using thin layer chromatography (Lower).