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bamDR197L restores the OM permeability barrier in a bamAE373K mutant. Serial 10-fold dilutions of stationary-phase cultures of the strains indicated were prepared in 96-well plates and then were spotted onto LB medium alone or onto LB medium supplemented with 625 μg/mL bacitracin, 60 μg/mL vancomycin, or 0.5% SDS + 1.0 mM EDTA and were incubated overnight at 37 °C.
