Fig. 3.

Oxygen consumption by Cox2 and the supercomplex in the presence of decylubiquinol and DTT. Reactions were recorded using an oxygen electrode at 40 °C. (A) Oxygen consumption by Cox2. The reaction was inhibited by 1 mM potassium cyanide. (B) Oxygen consumption by the supercomplex. This reaction was not inhibited by 40 μM stigmatellin, but inhibited by 1 mM potassium cyanide. (C) Oxygen consumption by the supercomplex. This reaction could be accelerated by addition of air oxidized horse heart cytochrome c (60 μM). The cytochrome c has to be injected together with the protein to avoid direct reduction by DTT. The scale bars indicate a change in the dioxygen concentration by micromole per liter per minute.