Fig. 4.

The effect of increasing decylubiquinol concentrations on the supercomplex activities. All reactions were measured spectrophotometrically at 80 °C. (A) Decylubiquinol oxidation in the presence (dashed line) and absence (solid line) of 1 mM potassium cyanide. (B) Cytochrome c reduction (dashed line) and decylubiquinol oxidation (solid line) in the presence of 60 μM horse heart cytochrome c and 1 mM potassium cyanide. (C) Cytochrome c reduction (dashed line) and decylubiquinol oxidation (solid line) in the presence of 60 μM horse heart cytochrome c. Error bars indicate the standard errors of the mean from three experiments. The y axis shows the enzyme-specific activity in percent.