Fig. 5.

Cytochrome c reduction of Cox2 in the presence of 1 mM potassium cyanide and 45 μM decylubiquinol. The reaction was measured spectrophotometrically at 80 °C as described in SI Materials and Methods for cytochrome c reduction. A specific activity of 0.53 μmol/ min per milligram was calculated for the Cox2 ubiquinol∶cytochrome c oxidoreductase activity. For the blank measurements buffer without Cox2 was used.