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. 2011 Dec 7;63(5):1863–1871. doi: 10.1093/jxb/err363

Fig. 3.

Fig. 3.

Comparative expression of MVA and MEP pathway-specific genes in rubber tree clones varying in latex carotenoid content. (a) Ultracentrifugation of RRIM 600 and PB 235 latex produced separated zones viewed from the side of the centrifuge tubes. PB 235 latex contains more carotenoids as shown by the more intense yellow-orange zones. (b) Latex from clones RRIM 600 and PB 235 contain the same amount of rubber based on total solids content. (c) Expression trends of gene transcripts specific to the MVA (HMGR, HMGS, PMK, MK) and MEP (DXS, DXR) pathways in tree clones RRIM 600 and PB 235. Both clones showed the same expression trends using latex RNAs from two biological replicates (see Supplementary Fig. S1 at JXB online). Av, average; SD, standard deviation; HMGS, hydroxymethyglutaryl coenzyme A synthase; HMGR, hydroxymethyglutaryl coenzyme A reductase; MK, mevalonate kinase; PMK, phosphomevalonate kinase; DXS, 1-deoxy-D-xylulose 5-phosphate synthase; DXR, 1-deoxy-D-xylulose 5-phosphate reductoisomerase.