Table 1.

Statistics of sequence assemblies

	Number of sequences	Total length (nt)
Sanger sequences for assembly	34 152
Sanger UTs (≥50 nt)	8 618	5 415 737
Consensi	3 999
Singletons	4 619
NG contigs (≥100 nt)	29 751	5 594 713
Input sequence for hybrid assembly
(Sanger UTs + NG contigs)	38 369
Hybrid contigs	23 257	8 078 653
Consensi	5 924
Singletons	17 333

Assembly of Sanger sequences and next generation (NG) reads resulted in 8618 unique transcripts (UTs) and 29 751 NG contigs, respectively. The rationale of hybrid assembly (23 257 contigs) was to combine the benefits of Sanger (longer transcript assemblies) and NG (deeper sequence coverage) sequencing methods so that a significantly more comprehensive latex transcriptome could be obtained.