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. 2011 Dec 8;63(5):1937–1949. doi: 10.1093/jxb/err388

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — DOF6 expression patterns. DOF6 mRNA levels relative to UBC21 in: (A) wild-type plants in different plant organs (L, adult rosette leaves; F, flowers; R, roots; S, pool of developing siliques from 4 to 15 days after pollination (dap); DS, dry seeds); (B) siliques at different dap and (C) seeds at different hours after imbibition (hai). Means and standard errors for three replicates are shown. (D) Expression of the GUS reporter gene driven by the DOF6 promoter in seeds imbibed for 3 h. Seed covers were removed before incubating with the staining solution. (E, F) In situ hybridization of DOF6 transcripts in Col-0 seed sections. Seeds were imbibed for 24 h and hybridized using antisense (E) and sense (F) DOF6 probes. Gene-specific mRNA levels are relative to UBC21. Bars, 100 μm (D), 100 and 50 μm (E and F).