Fig. 4.
ABA effect on DOF6 expression and ioexDOF6 seed germination. (A and B) DOF6 expression by quantitative RT-PCR in freshly harvested (FH; A) and after-ripened (AR; B) Col-0 seeds imbibed in water with 2 and 5 μM ABA, respectively. (C and D) Germination of FH (C) and AR (D) wild-type (WT) and ioexDOF6 seeds in water supplemented with 50 μM estradiol and 0.5 and 1 μM ABA, respectively. Germinating percentages are represented as the mean±standard error from three replicates. Germination patterns were confirmed in two different seed batches. Gene-specific mRNA levels are relative to UBC21.