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. 2011 Dec 23;63(5):2007–2024. doi: 10.1093/jxb/err400

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 14. — Imaging of NO production in barley embryogenic suspension cultures with 4,5-diaminoflorescein diacetate (DAF-2DA)-specific probe and confocal analysis. (A–D) Micrographs showing the differential interference contrast microscopy (DIC) image and the NO-dependent DAF-2DA fluorescence in green (maximum projection of several optical sections); (A) control cell showing a faint NO signal; (B) high NO production in stress-treated cells; (C) control cell treated with the NO donor GSNO, exhibiting a high NO signal; (D) negative control: stress-treated cells incubated with cPTIO, NO scavenger; bars, 45 μm. (E) Histogram showing relative fluorescence intensities reflecting differences between treatments. Stress-t cell, stress-treated cells. Letters indicate significant difference at P < 0.001 according to Duncan’s multiple-range test. (This figure is available in colour at JXB online.)