Table 2.

Kinetic parameters for PdxB using the coupled assay

substrate	kcat (s-1)	Km (μM)	kcat/Km (M-1s-1)	Intracellular concentration	Relative flux through PdxB
4PE	1.4 ± 0.32	2.9 ± 1.3	(6.7 ± 2.8) × 106	n.a.	n.a.
αKG	1.0 ± 0.24	93 ± 19	(1.1 ± 0.24) × 104	0.1 – 0.9 mM*	1.1 – 9.9 s-1
OAA	1.1 ± 0.31	86 ± 19	(1.3 ± 0.21) × 104	0.15 – 0.25 mM‡	2.0 – 3.3 s-1
pyruvate	1.3 ± 0.14	128 ± 20	(9.9 ± 0.16) × 103	0.4 mMˆ	4.0 s-1

^*(22);

^‡(23);

^ˆ(24)