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. Author manuscript; available in PMC: 2012 Mar 6.
Published in final edited form as: Breast Cancer Res Treat. 2010 Jan 7;124(1):89–99. doi: 10.1007/s10549-009-0716-3

Figure 6. Anti-miR-128a transfection sensitizes T+LET R lines to the growth inhibitory effects of TGFβ.

Figure 6

A) Transient transfection of anti-miR-128a inhibitor versus NC was performed to determine expression of miR-128a by real-time PCR, relative to U6 expression. Student’s t-test compared NC and anti-miR-128a transfected cells, with ** indicating statistical significance at a p-value < 0.01. B) Expression of TGFβR1 was quantified using real-time PCR, after transient transfection of NC or anti-miR-128a inhibitor. C) Transient transfection of NC or anti-miR-128a inhibitor was performed at concentrations of 10, 30 and 50nM and all conditions were treated with 1ng/ml TGFβ1 for 5 days. MTT assays for cell viability were performed in the MCF-7aro and T+LET R lines. For statistical analysis, Student’s t-test compared anti-miR-128a with NC transfected cells in 6C, with * indicating statistical significance at a p-value < 0.05.