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. Author manuscript; available in PMC: 2012 Mar 6.
Published in final edited form as: J Am Chem Soc. 2009 Aug 5;131(30):10753–10762. doi: 10.1021/ja900296u

Table 1.

Steady-state kinetic parametersa for nitrocefin, imipenem, cefaclor, and meropenem hydrolysis by as-isolated Bla2 containing 1 equivalent of Zn(II).

Substrate: Nitrocefin Substrate: Imipenem Substrate: Cefaclor Substrate: Meropenem
Km (μM) 25 ± 4 89 ± 35 67 ± 6 110 ± 42
kcat (s-1) 41 ± 3 92 ± 21 24 ± 1 49 ± 11
a

Kinetic assays were conducted at 25 °C in 50 mM Hepes, pH 6.5.