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. Author manuscript; available in PMC: 2012 Mar 6.
Published in final edited form as: J Am Chem Soc. 2009 Aug 5;131(30):10753–10762. doi: 10.1021/ja900296u

Table 2.

Steady-state kinetic parameters with nitrocefin for Bla2 after incubation of the apoenzyme with 1 or 2 eq of Zn(II) or Co(II).

1Co-Bla2 CoCo-Bla2 1Zn-Bla2 ZnZn-Bla2
Km(μM) 18 ± 2 30 ± 9 28 ± 4 35 ± 4
kcat (s-1) 19 ± 1 47 ± 5 32 ± 2 42 ± 2

All experiments were conducted at 25 °C in 50 mM Hepes, pH 6.5. The Bla2 analogs were prepared by adding the indicated equivalents of metal to apo-Bla2 before the assays were conducted.