Figure 1. Domain structure of Shisa and Shisa-like proteins.

These proteins usually have a signal peptide (labeled as ‘SignalP’), an N-terminal cysteine-rich domain with a distinct cysteine pattern (‘C*C*CC*C*CC*C’ for Shisa proteins and ‘C*C*CC*CC’ for Shisa-like proteins), a single predicted transmembrane segment (labeled as ‘TM’) and a C-terminal proline-rich low complexity region predicted to be largely disordered. Cysteines (labeled as ‘C’) and positively charged residues (labeled as ‘R/K’) frequently occur at the C-terminal end of the predicted TM.