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. Author manuscript; available in PMC: 2012 Aug 3.
Published in final edited form as: Cell. 2012 Feb 3;148(3):583–595. doi: 10.1016/j.cell.2011.12.022

Figure 1. Characterization of DopR-Tango in vitro and in Drosophila.

Figure 1

(A) Design of the DopR-Tango transgene; note HA epitope tag on LexA.

(B) Schematic illustrating DopR-Tango mechanism.

(C) DopR-Tango reporter (β-gal) activity in response to indicated ligands in HEK293 cells co-transfected with CMV-GAL4, UAS-DopR-Tango and LexAop-β-gal. Increases in β-gal activity relative to background are shown. Error bars represent the standard error of mean (SEM). Asterisks represent statistically significant increases (p<0.05, t-test with Bonferroni correction, n=3).

(D) Representative confocal projections of whole-mount brains from DopR-Tango flies visualized with GFP native fluorescence (green) and anti-HA immunostaining (magenta).