Table 1.
Summary of results obtained from thermal shift assays for the identification of GbpA- and HbpA2-family ligands out of a test set of typically family 5 SBP allocrites.
| GbpAHi | GbpAAp | GbpAHp | GbpAPm | HbpA2Hp | HbpA2Ap | |
|---|---|---|---|---|---|---|
| Tm (°C)a | ||||||
| GSSG | 17.5 | 14.0 | 16.0 | 20.5 | - | - |
| GSH | 12.5 | 11.0 | 14.0 | 13.0 | - | - |
| S-methylglutathione | 10.5 | 7.5 | 11.5 | 11.5 | - | - |
| S-nitrosoglutathione | 7.0 | 6.5 | 9.5 | 12.0 | - | - |
| glutathione cysteine disulfide | 10.0 | 6.0 | 7.0 | 12.0 | - | - |
| S-hexylglutathione | - | - | 4.5 | 6.5 | - | - |
| S-decylglutathione | - | - | 2.5 | 3.0 | - | - |
| homoglutathione γ-glutamylcysteine cysteinylglycine glycylleucine glycylglycylcysteine δ-aminolevulinic acid proline-betaine nickel} |
- | - | - | - | - | - |
aInteracting ligands are identified by means of the respective ΔTm-values, the apparent Tm-differences in °C for the ligand-protein complexes relative to the uncomplexed proteins. A negative sign indicates ligands for which the ΔTm is smaller than 1.5°C.