Table 2.

Summary of the dissociation constants for the interaction of our GbpA-family test set with the physiologically relevant glutathione forms (GSSG and GSH), and the artificial S-methylglutathione (S-me-GSH) as determined by ITC at 37°C.

	GbpAHia	GbpAAp	GbpAHp	GbpAPm
	Kd (μM)
GSSG	12.9 ± 0.3	0.33 ± 0.05	2.1 ± 0.2	0.15 ± 0.03
GSH	56.4 ± 3.0	1.58 ± 0.2	1.9 ± 0.1	0.26 ± 0.05
S-methylglutathione	212 ± 17	1.17 ± 0.05	0.90 ± 0.07	0.55 ± 0.04

^aValues were taken from ref. [8].