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Transcription analysis of the co-overexpressed MUT pathway genes DAS1/FLD1/TKL1 in HRP/CalB overexpressing strains. The transcript levels were normalized to the corresponding transcript levels of ARG4. The increase in AOX2 transcript levels indicated successful induction with methanol. All co-overexpression strains showed elevated transcript levels of the respective target mRNAs compared to the benchmark strains.
