Table 1.
reporter enzyme | MUT enzyme | RQ |
qs [mmol·g-1·h-1] |
qp [U·g-1·h-1] |
η [U·mmol-1] |
vol. productivity [U·l-1·h-1] |
---|---|---|---|---|---|---|
HRP | benchmark | 0.54 | - 1.67 | 1.66 | 0.99 | 38.79 |
DAS1 | 0.56 | - 0.51 | 1.56 | 3.06 | 28.35 | |
FLD1 | 0.57 | - 1.22 | 2.01 | 1.65 | 49.11 | |
TKL1 | 0.57 | - 0.88 | 1.96 | 2.22 | 39.87 | |
CalB | benchmark | 0.57 | - 1.32 | 1.39 | 1.05 | 30.27 |
DAS1 | 0.57 | - 0.36 | 0.78 | 2.15 | 15.23 | |
FLD1 | 0.57 | - 0.70 | 1.49 | 2.13 | 31.27 | |
TKL1 | 0.57 | - 0.35 | 0.12 | 0.34 | 2.07 |
The respiratory quotient (RQ), the specific substrate uptake rate (qs), the specific productivity (qp), the efficiency factor (η) and the volumetric productivity of the benchmark strains (overexpressing either HRP or CalB) and the respective benchmark strains co-overexpressing either of the MUT pathway enzymes DAS1, FLD1 or TKL1 were determined