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. 2012 Jan 24;13:37. doi: 10.1186/1471-2164-13-37

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Copyright ©2012 Hansen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Synteny comparison and sequence alignment of spf and VSsrna24. (A) The order of genes that flank spf and other sRNA genes (filled arrows) in A. salmonicida and E. coli are shown. Question marks indicate genes with unknown function. (B) Sequence alignment of spf and VSsrna24 from selected members of Vibrionaceae, E. coli and Serratia marcescens. The CRP binding site of E. coli, and -10 and -35 promoter regions in front of spf and VSsrna24 are based on knowledge from E. coli [7,8]. The vertical open arrows denote the 5' end of Spot 42 and VSsrna24 based on 5' RACE data on A. salmonicida presented in this work. The horizontal arrows (#1-4) denote areas deleted in the spf (#1-2) and VSsrna24 (#3-4) deletion strains. Numbers in parenthesizes denote numbers of nt to the nearest CDS. Asterisks indicate invariable positions.