Table 1.

Amplification, sequencing and site-directed mutagenesis primers for RNase 8

Gene-amplification
RN8F	5'- CAC ATT GCC CTG CAA TAA CTG GCT -3'
RN8R	5'- AGG TTG AGT GTG TGG GAG GGA AAT -3'
Sequencing
RNase 8 Fi	5'- TCA CTC TGT TCC ACT GTC TCC CTT -3'
RNase 8 Ri	5'- AGT CTG CAG AGT GTG AG GTG GAA -3'
Human RNase 8 nested RACE (human spleen cDNA)
rcRN8 (3' → 5')	5'-CAA CTT TAT CCA AGT GCA CAG GA-3'
R85Rn (3' → 5')	5'-CTG GGT CAC CCT GTT GTG GAG-3'
Human RNase 8 nested reverse-trancriptase PCR (RT-PCR)
R8S	5'-ACA ATA GAA TGC CAG GGG TGT TCA-3'
R85R	5'-CAA CTT TAT CCA AGT GCA CAG GA-3'
R8Sn	5'-CAT TGC CCT GCA ATA ACT GGC TT-3'
R85Rn	5'-CTG GGT CAC CCT GTT GTG GAG-3'
Human RNase 8 quantitative RT-PCR
RNase 8fx	5'- CAT AGC CTG CAA GAA TAG CTG TAA AA-3'
RNase 8rx	5'- TCA CCC ATG GTC AGG GAC AT-3'
6FAM-RNase 8-TAMRA	6FAM-CTG CCA CCA GAG CCA CGG GC-TAMRA
Nested primate genome RNase 8
5'R8F	5'-GCC AGG GGT GTT CAA TAT CTT AG-3'
3'R8R	5'-AGG TTG AGT GTG TGG GAG GGA AAT-3'
5'R8Fn	5'-CAC ATT GCC CTG CAA TAA CTG GCT-3'
3'R8Fn	5'-AGT CTG CAG AGT GTG AGG TGG AAC-3'
RNase 8 expression plasmids
CCC3
R8D	5'-AAG CTT ATT GCC CTG CAA TAA CTG GCT TAG GGT-3'
R8C	5'-CTC CTC CTC TCT AGA GAC AAC TTT ATC CAA GTG CAC A-3'
CCC4
R8O	5'-AAG CTT ACT CTG TTC CAC TGT CTC CCT TAA GAG AG-3'
R8C	5'-CTC CTC CTC TCT AGA GAC AAC TTT ATC CAA GTG CAC A-3'
CCC5 mutagenesis
R85F	5'-TCT CCC TTA AGA GAG ATA GCA CCG GCC AGA GCA-3'
R85R	5'-TGC TCT GGC CGG TGC TAT CTC TCT TAA GGG AGA-3'