Table 1.
Transgenic line | Copy number | Genomic insertion locus (scaffold:position)* | Score | E- value | Transcript |
---|---|---|---|---|---|
N66-2 | 1 | 3:16,990,223 | 1132.0 | 0 | POPTR_0003s17690 (no functional annotation) |
N66-5 | 1 | 3:15,414,346 | 1222.2 | 0 | POPTR_0003s15650 CTP synthase (UTP-ammonia lyase) |
*based on BLAST-results against the genome sequence of P. trichocarpa v2.0 (Phytozome v7.0; http://www.phytozome.net/poplar). Successful positioning of blasted sequence on the physical map of P. trichocarpa was assigned to the Populus-aspen genome because of the high collinearity between the P. trichocarpa and P. tremula/P. tremuloides genomes [49].