Table 2.
Copy number of ATDs-rolC in the double transgenic lines, and genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.
| Transgenic line | Copy number | Genomic insertion locus (scaffold:position)* | Score | E-value | Transcript (POPTR_) |
|---|---|---|---|---|---|
| N82-2 | 1 | 18:5,952,389 | 841.7 | 0 | --- |
| N82-3 | 1 | 7:3,347,471 | 854.3 | 0 | 0007s05190 no functional annotation |
| N82-4 | 1 | 8:2,414,980 | 580.2 | 5.0e-164 | 0008s04320 Cyclin, N-terminal domain |
| N82-5 | 1 | 607:11,508 | 132.9 | 1.4e-29 | 0607s00230 no functional annotation |
| 18:4,994,998 | 165.4 | 1.7e-39 | 0018s05440 Ankyrin Repeat-Containing |
||
| N82-7 | 4 | 7:9,643,105 | 71.6 | 2.5e-11 | --- |
| 2:14,852,606 | 41.0 | 2.2e-2 | --- | ||
| n.d.** | |||||
| N82-8 | 1 | 18:1,568,406 | 1463.8 | 0 | POPTR_0018s01710 |
| or | F-Box/Leucine Rich Repeat Protein | ||||
| 3:13,577,759 | 1551.2 | 0 | POPTR_0003s13180 Heat Shock Protein 90 | ||
| N82-10 | 2 | 2:10,740,018 | 612.2 | 5.3e-174 | 0002s14460 Zinc-finger double stranded |
| 5:9,290,567 | 91.5 | 3.4e-17 | 0005s12520 GRAS family transcription factor |
||
| N82-11 | 1 | 16:13,606,858 | 879.5 | 0 | POPTR_0016s14360 Elongation factor P, C-terminal |
| N82-12 | 2 | 17:7,632,882 | 44.6 | 9.8e-3 | --- |
| n.d.** | --- | ||||
| N82-14 | 2 | 3:13,408,914 | 322.3 | 1.9e-89 | --- |
| n.d.** | --- | ||||
| N82-15 | 1 | 2:21,183,733 | 199.7 | 6.8e-50 | --- |
| N92-1 | 1 | 15:6,453,477 | 286.2 | 1.6e-75 | --- |
| N92-2 | 1 | 2:10,740,357 | 706.4 | 0 | --- |
| N92-3 | 1 | 11:16,469,472 | 39.2 | 2.0e-3 | POPTR_0011s13850 (COBRA-like protein) |
| N92-4 | 1 | 10:15,737,708 | 515.2 | 2.1e-144 | --- |
| N92-5 | 1 | 10:19,481,904 | 417.9 | 4.4e-115 | --- |
| N92-6 | n.d.** | n.d.** | |||
| N95-1 | 1 | n.d.** | |||
| N95-2 | 2 | 25:155,725 | 1490.9 | 0 | POPTR_0025s00400 (CYTH domain) |
| 15:824,499 | 48.2 | 4.2e-4 | --- | ||
| N95-3 | 1 | 9:9,143,423 | 291.6 | 3.0e-77 | POPTR_0009s10720 (Arylacetamide deacetylase) |
| N95-4 | 1 | 17:7,632,882 | 44.6 | 3.2e-3 | --- |
| N95-5 | 1 | 16:7,726,062 | 562.1 | 1.4e-158 | --- |
| N95-6 | n.d.** | n.d.** | |||
In bold: blast-results with high e-values. In BLAST-analyses where more than one hit was given, either the one with lower e-value or when similar, both hits are shown.
*based on BLAST-results against the genome sequence of P. trichocarpa v2.0 (Phytozome v7.0; http://www.phytozome.net/poplar). Successful positioning of blasted sequence on the physical map of P. trichocarpa was assigned to the Populus-aspen genome because of the high collinearity between the P. trichocarpa and P. tremula/P.tremuloides genomes [49].
**n.d. = not determined.