Table 4.
Pronounced phenotypes of ATDs tagged poplar lines: annotation results of new ATDs flanking sequences.
| Transgenic line | Number of variants | Variant affiliation | Phenotype | Genomic insertion locus (scaffold:position)* | Score | E-value | Transcript (POPTR_) | Functional annotation |
|---|---|---|---|---|---|---|---|---|
| N82-2-11 | Variegated leaf | n.d.* | ||||||
| N82-2-13 | Variegated leaf | 18:5,952,981 | 154.6 | 1.5e-35 | ||||
| N82-2 | 5 | N82-2-14 | Dwarf plant, weakly shriveled leaf | 18:5,946,821 | 253.8 | 3.6e-66 | 0018s06220 | 2OG-Fe(II) oxygenase superfamily |
| N82-2-64 | Shriveled leaf | 1599:3,445 | 250.2 | 3.8e-65 | ||||
| N82-2-70 | Wavy leaf | 3:15,139,843 | 702.8 | 0 | 0003s15320 | Hypothetical protein (basic region leucine zipper) | ||
| N82-3-23 | Serrated leaf | 10:20,130,280 | 206.9 | 5.7e-52 | 0010s23600 | 1,4-alpha-glucan branching enzyme/starch branching enzyme II | ||
| N82-3 | 3 | N82-3-37 | Serrated leaf | 18:8,430,672 or | 1063.5 | 0 | 0018s07730 | No functional annotation for this gene |
| 15:1,812,282 | 1059.9 | 0 | 0015s02530 | Myosin VII | ||||
| N82-3-66 | Shriveled leaf | 7:3,447,049 | 311.5 | 9.1e-83 | 0007s05350 | Histone binding protein RBBP4 | ||
| N82-5-3 | Lanceolated leaf | 14:11,531,333 | 450.3 | 1.6e-124 | ||||
| N82-5 | 4 | N82-5-20 | Necrotic leaf | 14:2,480,856 | 302.4 | 9.4e-81 | ||
| N82-5-26 | Bonsai plant | n.d.* | ||||||
| N82-5-28 | Bonsai plant | 607:2,304 | 448.5 | 3.7e-124 | 0607s00200 | No functional annotation for this gene | ||
| N82-7 | 1 | N82-7-1 | Shriveled leaf | 11:13,627,432 | 565.7 | 1.7e-159 | 0011s10950 | Protein of unknown function (DUF_B2219) |
| N82-11-1 | Crippled growth in vitro, saw toothed leaf | 16:3,641,919 | 280.8 | 1.5e-73 | 0016s05700 | Zinc ion binding; nucleic acid binding | ||
| N82-11 | 3 | N82-11-4 | Pale green leaf | n.d.* | ||||
| N82-11-5 | Pale green leaf | 16:13,607,077 | 426.9 | 1.4e-117 | 0016s14360 | EF-P Elongation factor | ||
| N82-14-2 | Lanceolated leaf | 4:23,008,963 or | 780.3 | 0 | 0004s24320 | Protein of unknown function (DUF1218) | ||
| 17:944,412 | 1115.8 | 0 | 0017s01360 | Apoptotic ATPase | ||||
| N82-14-3 | Shriveled leaf | 14:6,540,903 | 904.8 | 0 | 0014s08850 | Glycosyl hydrolases family 18; Pt-CHI3.5 | ||
| N82-14-4 | Weakly serrated leaf | 11:1,379,364 or | 535.1 | 1.2e-150 | ||||
| N82-14 | 7 | 3:13,395,264 | 1079.7 | 0 | 0003s12970 | No functional annotation for this gene | ||
| N82-14-5 | Shriveled leaf | 10:14,732,583 | 892.2 | 0 | 0010s15550 | No functional annotation for this gene | ||
| N82-14-6 | Weakly shriveled leaf | 2:13,782,413 | 298.8 | 1.1e-79 | ||||
| N82-14-9 | Shriveled leaf | 14:10,351,781 | 1090.5 | 0 | 0014s14140 | Protein of unknown function (DUF3754) | ||
| N82-14-10 | Undulating leaf | 17:1,256,405 | 1204.1 | 0 | ||||
| N82-15-6 | Bushy plant | 4:9,615,348 | 1577.4 | 0 | 0004s11010 | Pentatricopeptide repeat-containing protein | ||
| N82-15 | 2 | N82-15-10 | Lanceolate, serrated | 17:2,279,602 or | 226.7 | 5.9e-58 | 0017s03150 | No functional annotation for this locus |
| leaf | 14:14,660,942 | 226.7 | 5.2e-58 | |||||
| N92-1 | 2 | N92-1-1 | Variegated leaf | 10:15,124,072 | 147.4 | 6.0e-34 | 0010s16070 | Stress responsive A/B Barrel Domain |
| N92-1-6 | Variegated leaf | 10:15,114,170 | 206.9 | 1.2e-51 | ||||
| N95-1 | 1 | N95-1-1 | Variegated leaf | n.d.* | ||||
| N95-3 | 1 | N95-3-1 | Variegated leaf | n.d.* | ||||
Genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.
In BLAST-analyses where more than one hit was given, either the one with lower e-value or when similar both hits are shown.
*n.d. = not determined.